Composite

Part:BBa_K1813063

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-09-11)

ptac pelB AgCPR pelb CYP2D6

With ptac powering the transcription of BBa_K1813053 and BBa_K1813055, this part allows the co-localization of large amounts of simultaneously expressed CYP2D6 ( BBa_K1813005) and A. gambiae NADPH P450 reductase ( BBa_K1813010) to the bacterial periplasm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2783
    Illegal BamHI site found at 626
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 2448
    Illegal NgoMIV site found at 3373
    Illegal AgeI site found at 424
    Illegal AgeI site found at 1606
    Illegal AgeI site found at 1762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2122


[edit]
Categories
Parameters
n/aptac pelB AgCPR pelb CYP2D6